Figure 2.

Amiloride (Ami) inhibited endoplasmic reticulum stress-induced X-box binding protein 1 (XBP1) splicing. (A) Cells were treated with Ami (200–500 µM) for 1 h and then stimulated with tunicamycin (Tm: 0.01 µg·mL⁻¹) or dithiothreitol (DTT: 3 mM) for 6 h. Reverse transcription-polymerase chain reaction was performed using specific primers for XBP1 and GAPDH. (B) Cells were treated with amiloride (Ami: 200 µM) for 1 h and then stimulated with tunicamycin (Tm: 0.01 µg·mL⁻¹) or dithiothreitol (DTT: 3 mM) for 18 h. Western blotting was performed using anti-XBP1 (54 kDa) and anti-GAPDH (37 kDa) antibodies. Cont, control.