Figure 7.

Effect of amiloride (Ami) on endoplasmic reticulum stress-induced cell death. (A) and (B) Cells were treated with Ami (200 µM) for 1 h and then stimulated with tunicamycin (Tm: 0.1 µg·mL⁻¹) or dithiothreitol (DTT: 3 mM) for the periods indicated. Lactate dehydrogenase (LDH) activity was measured as an indicator of cytotoxicity. (C) and (D) Cells were treated with Ami (20 µM) for 1 h and then stimulated with Tm (0.1 µg mL⁻¹) or DTT (3 mM) for the indicated times. *P < 0.05, **P < 0.01 Tm or DTT versus Ami + Tm or Ami + DTT (n= 3–8). Cont, control.