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. Author manuscript; available in PMC: 2010 Feb 20.
Published in final edited form as: Chem Res Toxicol. 2008 Feb 14;21(4):787–795. doi: 10.1021/tx7004508

Figure 2.

Figure 2

ESI+-MS spectra and deconvoluted spectra (inset) of N7G-PBA-Cl and N7G-EMA-Cl treated AGT protein and its variants. (A) Wild type protein: A = unmodified AGT (calculated M = 21 876 Da, observed M = 21 880 Da), B = AGT containing a single chlorambucil cross-link to guanine (calculated M = 22 259 Da, observed M = 22 263 Da), C = AGT containing two chlorambucil cross-links to guanine (calculated M = 22 642 Da, observed M = 22 646 Da). (B) C145A AGT variant: A = unmodified C145A AGT (calculated M = 23 015 Da, observed M = 23 017 Da), B = C145A AGT containing a single chlorambucil cross-link to guanine (calculated M = 23 398 Da, observed M = 23 401 Da). (C) C145A/C150S variant: A = unmodified C145A/C150S AGT (calculated M = 22 996 Da, observed M = 23 001 Da), B = C145A/C150S AGT containing a single chlorambucil cross-link to guanine (calculated M = 23 379 Da, observed M = 23 384 Da). (D) Wild type protein: A = unmodified AGT (calculated M = 21 876 Da, observed M = 21 876 Da), B = AGT containing an intramolecular mechlorethamine cross-link (calculated M = 21 959 Da, observed M = 21 959 Da), C = AGT containing a single mechlorethamine cross-link to guanine (calculated M = 22 110 Da, observed M = 22 110 Da), D = AGT containing two mechlorethamine cross-links to guanine (calculated M = 22 344 Da, observed M = 22 345 Da).