FIGURE 6.

Evidence suggests functionally relevant intersubunit interactions between G156D and N160R. Shown is a representative inside-out patch clamp recording from COSm6 cells transfected with cDNA of SUR1 and a mixture of G156D Kir6.2 and N160R Kir6.2 at equal molar ratio. Patch was exposed to 1 mm ATP or 5 μm oleoyl-CoA as indicated. Of note, neither G156D Kir6.2 nor N160R Kir6.2 alone generated measurable currents when co-expressed with SUR1 (n = 10–15; not shown) (31). Also, placing G156D and N160R in the same subunit failed to generate potassium currents upon co-expression with SUR1 (Table 1).