TABLE 1.
Adenosine and inosine uptake and analog toxicity by a panel of cfnt2-K155 mutants in L. donovani Δldnt1/Δldnt2
| Cell line | Adenosine, uptakea | Inosine uptakeb | Tubercidin EC50c | Formycin B EC50c |
|---|---|---|---|---|
| Vector | − | − | 89.1 ± 13 | 18.9 ± 4.9 |
| CfNT1 | ++++ | − | 0.0139 ± 0.0046 | 1.18 ± 0.14 |
| CfNT2 | − | ++++ | 3.23 ± 1.2 | 0.0163 ± 0.0064 |
| cfnt2-K155N | + | ++ | 0.733 ± 0.23 | 0.363 ± 0.11 |
| cfnt2-K155A | ++ | + | 0.610 ± 0.20 | 0.0485 ± 0.018 |
| cfnt2-K155Q | +/− | + | 0.773 ± 0.30 | 0.0828 ± 0.032 |
| cfnt2-K155R | + | +++ | 5.74 ± 1.3 | 0.185 ± 0.085 |
| cfnt2-K155T | − | + | 1.62 ± 0.61 | 0.102 ± 0.037 |
| cfnt2-K155E | − | − | 1.90 ± 0.41 | 0.322 ± 0.11 |
| cfnt2-K155M | − | − | 3.01 ± 1.2 | 0.102 ± 0.043 |
| cfnt2-K155Y | − | − | 6.67 ± 0.91 | 2.41 ± 1.0 |
| cfnt2-K155L | − | − | 110. ± 16 | 33.6 ± 5.4 |
a Uptake of 5 μm [3H]adenosine relative to vector cells (set to “−”), n = 2.
b Uptake of 5 μm [3H]inosine relative to uptake in the presence of 1 mm unlabeled inosine, n = 2.
c EC50 values are expressed in μm (n = 3–5).