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. 2009 Dec 26;285(9):6053–6062. doi: 10.1074/jbc.M109.082990

FIGURE 3.

FIGURE 3.

Overexpression of Bcl-2 protects against apoptosis induced by STS in T-REx-293 cells overexpressing native mVDAC1 but not E65Q-, E72Q- or E202Q-mVDAC1 mutants. shRNA-T-Rex-293 cells were transiently cotransfected with plasmids pEGFP-Bcl-2 or pEGFP and pcDNA4/TO, respectively, encoding native, E65Q-, E72Q-, or E202Q-mVDAC1 (or pcDNA4/TO as control) and grown for 48 h in the presence of tetracycline (1 μg/ml, to induce expression) prior to exposure to STS (1.25 μm) for 4 h. Apoptosis was quantitatively analyzed by acridine orange/ethidium bromide staining. Data represent mean values ± S.E. (n = 3).