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. 2009 Dec 15;285(9):6071–6079. doi: 10.1074/jbc.M109.069542

FIGURE 7.

FIGURE 7.

Aβ40 FOs seed the formation of FOs from monomers. Aβ40 fibrils and FOs were sonicated and incubated at 25 °C with 45 μm Aβ40 monomers. At the time indicated, aliquots were spotted onto nitrocellulose membrane and probed with the fibril- and FO-specific OC-like antibody LOC. A, Aβ40 monomers seeded with 2 and 5% of FOs formed LOC-immunoreactive aggregates within 1.5 h. In contrast, Aβ40 monomers in the absence of seeds developed LOC immunoreactivity after 1.5 h. Monomers seeded with fibrils developed LOC reactivity at 1.5 and 2 h. B, Aβ40 FO and fibril seeds control. Immunoreactivity of seeds diluted to 2% is below the limit of LOC detection. C, quantification of LOC immunoreactivity of Aβ40 monomers alone and monomers seeded with 2% FOs. The amount of LOC reactivity represents the amount of FOs or fibrils in each reaction. AU, arbitrary units.