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. 2009 Dec 23;285(9):6127–6133. doi: 10.1074/jbc.M109.074229

FIGURE 2.

FIGURE 2.

Sterol composition and squalene accumulation in hem1Δ mutants. A, wild type BY4742 (gray bar), hem1Δ + δALA (white bar), and hem1Δ + ergosterol + Tween 80 (black bar). B, QM dga1Δlro1Δare1Δare2Δ (gray bar), QMhem1Δ + δALA (white bar), and QMhem1Δ + ergosterol + Tween 80 (black bar). Cells were grown to the stationary phase, lipids were extracted, and sterols/squalene were quantified by GLC-MS. Data are mean values of five independent experiments. Error bars, S.D. values. *, in these samples, only marginal amounts of the respective compound were detected. SQ, squalene; ERG, ergosterol; LAN, lanosterol.