FIGURE 4.

Hepatic BI-1 overexpression increases glucose tolerance in mice fed a normal chow diet. a, immunoblot analysis was performed for BI-1 or GFP and actin on liver lysates from mice provided a normal chow diet and infected with Ad BI-1 or Ad GFP. Livers were collected from mice after an overnight fast, and proteins were extracted and processed as described under “Experimental Procedures.” Each lane represents liver lysates from a different mouse. b, blood glucose concentrations (mg/dl) in ND-fed mice immediately before and 24 and 120 h after adenovirus treatment are shown. The results are the mean ± S.E. of n = 9 mice per group (*, p ≤ 0.05; **, p ≤ 0.01). c, glucose tolerance tests (0.5 g/kg, intraperitoneal) were performed on ND-fed mice 60 h after adenovirus injection. Results are given as the mean ± S.E., n = 9 per group (**, p ≤ 0.01; ***, p ≤ 0.001). d, shown are G6P and actin protein levels in the liver of Ad BI-1- or Ad GFP-infected ND-fed mice as assessed by immunoblotting (n = 3 of each group).