FIGURE 5.

Adenovirus-mediated expression of BI-1 in the liver inhibits IRE-1α-mediated XBP-1 processing. a, shown is relative expression of spliced Xbp-1 (Xbp-1s), unspliced Xbp-1u, and the XBP-1 target Erdj4 in the liver of Ad BI-1-or Ad GFP-infected ND and HFD mice as measured by real-time PCR (n = 5 per group). b, shown are XBP-1s, GRP78, CHOP, and C/EBPα protein levels in the liver of Ad BI-1- or Ad GFP-infected ND- and HFD-fed mice as assessed by immunoblotting (n = 4–5 of each group). c, shown are XBP-1, GRP78, CHOP, and C/EBPα protein levels in the liver of Ad BI-1- or Ad GFP-infected db/db mice as assessed by immunoblotting (n = 7 of each group). d, shown is co-immunoprecipitation (IP) of BI-1 and IRE1α. HeLa cells with tetracycline (TL)-inducible HA-tagged BI-1 expression were either stimulated with (+) or without (−) doxycycline. BI-1 was immunoprecipitated using α-HA antibody, and its interaction with IRE1α was assessed by immunoblotting.