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. 2009 Dec 10;285(9):6208–6216. doi: 10.1074/jbc.M109.062208

FIGURE 7.

FIGURE 7.

Cross-reactivity of anti-Fbl region A and anti-ClfA region A antibodies. Microtiter wells were coated with rClfA221–559 (250 nm) (●) or rFbl206–533 (250 nm) (○). Increasing dilutions of polyclonal rabbit anti-Fbl region A antibodies (A) or polyclonal rabbit anti-ClfA region A (B) antibodies were added to the wells. Bound antibody was detected using peroxidase-conjugated goat anti-rabbit IgG antibody. The results shown are the mean values of triplicate samples. The error bars show the standard deviation. For surface plasmon resonance analysis of rClfA221–559 and rFbl206–533 binding to murine monoclonal antibody 12-9, the antibody was captured on the surface of a CM5 sensor chip by a rabbit anti-mouse Fc antibody. The same concentration (100 nm) of rClfA221–559 and rFbl206–533 were passed over the surface (C). Injections started at 130 s and ended at 430 s.