. 2009 Dec 10;285(9):6227–6234. doi: 10.1074/jbc.M109.063669

TABLE 1.

Phenotypic characterization of TLR2 mutants

Group	Mutants	Response to PAM₃CSK₄^a	Response to MALP-2^a	Expression^b	Binding of PAM₃CSK₄^c
				% of wild type	% of wild type ± S.E.
Apolar residues with side chains inside the lipid-binding pocket	F284K	++	++	ND^d	142 ± 12
	F295K	++	++	ND	89 ± 7,2
	I319D	−	+	136	3,4 ± 4
	F325K	−	−	89,4	65 ± 4,8
	L328K	−	−	70,6	46 ± 6
	L331K	+	+	74	82 ± 8,7
Residues on the surface near the entrance into the binding pocket	F322K	++	++	ND	76,8 ± 6,4
	Y323K	−	−	102	42 ± 4,8
	L324K	+	+	102	75 ± 8,4
	D327K	−	+	56,5	106 ± 8,5
Residues not in contact with either TLR1 or lipopeptide Pam₃CSK₄	F237D	+	+	1,2	ND
	F239K	++	++	ND	ND
	E246K	++	++	ND	ND
	T262K	++	++	ND	ND
	D263K	−	−	68	119 ± 9,7
	S265K	++	++	ND	ND
	T288K	++	++	ND	ND
	G291K	−	−	68,2	92 ± 5,6
	G293R	−	−	134	96 ± 4,8
	D301K	++	++	ND	ND
	V309K	+	+	3,2	ND
	E310K	++	+	ND	ND
	T311K	++	+	ND	ND
	S329K	++	++	ND	ND
	Y332K	++	++	ND	ND
	L334K	+	++	54,5	ND
Mutants not expressed at the cell surface	G307K	−	−	0	ND
Mutants not expressed at the cell surface	L317K	−	−	0	ND

^a Response of TLR2 mutants to PAM₃CSK₄. −, no response; +, intermediate response compared with wild type; ++, no significant difference between mutant and wild type.

^b The expression of TLR2 mutants at the surface of transiently transfected cells was measured by flow cytometry with an antibody that recognizes the FLAG epitope expressed at the N terminus of the protein. The results are expressed as the percentages of cells with fluorescence intensity above vector control compared with wild type.

^c Binding of fluorescent A₄₈₈-Pam₃CSK₄ to the cell surface was measured as described under “Experimental Procedures” and expressed as a percentage of wild type.

^d ND, not done.