Channel unitary conductances
Open channel conductances were determined in the cell-attached configuration. When few channels remained active in the patch, some pulses to −110, −125, −150, and −160 mV allowed the determination of unitary currents. For each potential, the unitary current amplitudes were obtained from Gaussian fit to amplitude histograms. Unitary conductance values were obtained from linear fits to the single channel current-voltage curves within the −110 to −160 mV voltage range (data are given as means ± S.E., n = 3). Unitary conductance for KAT1 and KAT2 channels are those reported by Hoshi (7) and Pilot et al. (19), respectively. pS, picosiemens.