FIGURE 4.

Knockdown of hUTP18, Pes1, L7, and L11 in colon cancer (RKO) cells. A, RKO cells were transfected with Pes1, hUTP18, or control siRNAs. Protein levels were determined by Western blotting, and ubiquitously expressed DDX6 served as a loading control. B, RKO cells were transfected with the indicated combinations of siRNAs. Protein levels were determined by Western blotting. C and D, RKO cells were transfected with combinations of siRNAs as in B. Knockdown of L11 and S7 was confirmed by quantitative reverse transcriptase PCR. Relative expression levels of L11 and S7 are normalized to glyceraldehyde-3-phosphate dehydrogenase expression.