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KS6ST generates sulfated Langerin ligands. Untreated (thin line) and sialidase-treated CHO cells (bold line) transfected with or without a KS6ST (CHST1, keratan sulfate Gal-6-sulfotransferase) expression vector was incubated with Langerin-Fc chimera precomplexed with Cy3-labeled anti-human Fcγ in TSA buffer on ice for 1 h. After washing, cells were analyzed by flow cytometry. Binding of Langerin-Fc in the presence of 5 mm EDTA was taken as negative controls (gray).
