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Binding of Langerin-Fc to mouse splenocytes. A, mouse splenocytes were incubated with Langerin-Fc chimera precomplexed with Cy3-labeled anti-human Fcγ with or without sialidase pretreatment. B, mouse splenocytes were double stained with FITC-labeled anti-CD3/Langerin-Fc and FITC-labeled anti-CD19/Langerin-Fc with or without sialidase pretreatment.
