Skip to main content
. 2009 Dec 27;285(9):6401–6411. doi: 10.1074/jbc.M109.064063

FIGURE 3.

FIGURE 3.

Role of TAK1 in proliferation of C2C12 myoblasts. A, C2C12 myoblasts (10,000 cells/well) were plated in 24-well plate and transfected with vector alone or TAK1 shRNA plasmid. Cells were selected in the presence of puromycin (1.5 μg/ml), and the proliferation of myoblasts was measured using the BrdUrd uptake method as described under “Experimental Procedures.” Data presented here show that transfection of C2C12 myoblasts with TAK1 shRNA significantly reduced its protein levels and inhibited proliferation. *, p < 0.01, values significantly different from control shRNA transfected cells. B, stable transfection of C2C12 myoblasts with a dominant negative TAK1 (dnTAK1) plasmid also inhibited the proliferation of C2C12 myoblasts. #, p < 0.01, values significantly different from control shRNA transfected myoblasts. C, knockdown of TAK1 using RNAi technique did not affect the percentage of apoptotic cells in C2C12 myoblasts assayed using the annexin V-EGFP apoptosis detection kit and fluorescence-activated cell sorting method.