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Microscopic analysis of c-Fos and EGFP-c-Fos intranuclear localization in fractionated cells. These experiments were carried out in parallel with those presented in Fig. 6 with HeLa cells seeded and grown on coverslips. Endogenous c-Fos was detected using sequentially the sc52 rabbit anti-c-Fos antibody and an Alexa 488-labeled anti-rabbit secondary antibody. The nuclei were stained with Hoechst 33342. All of the pictures in the EGFP channel were acquired using the same time exposure.
