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. 2009 Dec 31;285(9):6563–6572. doi: 10.1074/jbc.M109.097410

FIGURE 7.

FIGURE 7.

Effect of C43 and LY294002 on Akt/PKB and ERK1/2 phosphorylation in GEO and GEO-CR cells. A, GEO and GEO-CR cells, loaded with FITC-SP or with FITC-C43, were stimulated with 100 ng/ml IGF-1 and then solubilized as described under “Experimental Procedures.” Total cell lysates (50 μg of protein/sample) were blotted with pThr308-Akt/PKB or pERK antibodies and then reblotted with Akt/PKB or ERK1/2 antibodies. Membranes were revealed by ECL and autoradiography. WB, Western blot. B, GEO and GEO-CR cells, treated or not with 25 μm LY294002 for 60 min, as indicated, were exposed to 100 ng/ml IGF-1 for 5 min and then solubilized. Cell lysates (50 μg of protein/sample) were blotted with pThr308-Akt/PKB or pERK antibodies and with Akt/PKB or ERK1/2 antibodies. Blots were revealed by ECL and autoradiography. The autoradiographs shown are representative of four independent experiments. All the autoradiographs shown are representative of four independent experiments.