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. 2009 Dec 28;285(9):6595–6603. doi: 10.1074/jbc.M109.068502

FIGURE 5.

FIGURE 5.

4E-BP1 and 4e-BP2 silencing does not enhance PPARγ protein level. 3T3-L1 adipocytes were transfected with 7.5 nmol of scrambled (Scr) or Map4k4 and 1.87 nmol of (each) 4E-BP1 and 4e-BP2 siRNA. 72 h later, cell lysates were examined for PPARγ protein levels as well as for efficiency of 4E-BP1 and 4e-BP2 gene silencing by SDS-PAGE followed by Western blot (A) and densitometry analysis (B). *, p < 0.05 when compared with scrambled siRNA transfected samples by ANOVA. Densitometry is representative of three independent experiments.