FIGURE 7.

Rapamycin inhibits Map4k4 regulation of 4E-BP1 phosphorylation and PPARγ expression. 3T3-L1 adipocytes 4 days post-differentiation induction were transfected with 7.5 nmol of either scrambled (Scr) or Map4k4 siRNA. 72 h later, cells were treated with 20 nm of rapamycin for 0.5, 1, or 2 h. Cell lysates were examined for the appearance of the hypophosphorylated form (4E-BP1α), and changes in the phosphorylation levels of 4E-BP1 at Ser-64, Thr-69, and Thr-36/45 were analyzed using appropriate antibodies by Western blot (A) (representative blots) and densitometry (B) analysis (n = 5). *, p < 0.05 when compared with scrambled siRNA transfected samples by ANOVA.