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. 2009 Dec 28;285(9):6595–6603. doi: 10.1074/jbc.M109.068502

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FIGURE 8. — Adenovirus-mediated overexpression of Map4k4 decreases PPARγ protein level as well as mTOR (Ser-2448) phosphorylation in 3T3-L1 adipocytes. 4 days post-differentiation induction, 3T3-L1 adipocytes were infected with 40 μl (1.4 × 10¹² particles/ml) of either HA control (Ad-control) or 3HA-Map4k4-HA (Ad-Map4k4) adenovirus. 72 h post-infection, cell lysates were examined for PPARγ protein levels as well as Map4k4, HA, phospho-mTOR (Ser-2448), and total mTOR by Western blot (A) (representative blots) and densitometry (B) analysis (n = 4). *, p = 0.05 when compared with control adenovirus infected samples by ANOVA.