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. 2009 Dec 29;285(9):6623–6635. doi: 10.1074/jbc.M109.060640

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FIGURE 4. — MoBP-mediated Moco transfer to apoNR and interaction of Arabidopsis MoBPs with Cnx1-E. A, Moco transfer to apoNR has been monitored by use of the nit-1 reconstitution assay. MoBP-bound Moco has been added to the nit-1 extract without cofactor releasing treatment. Casein was used as a control. For details see “Experimental Procedures.” The average values were obtained in three duplicate experiments. Error bars, S.E. B, cross-linked and immunoblotted samples with horseradish peroxidase-streptavidin conjugate (α-Biotin) and Cnx1-E as bait protein. Label transfer from Cnx1-E to the particular Arabidopsis MoBPs is shown in lane I for (B) MoBP1, (C) MoBP2, (D) MoBP3, (E) MoBP4, (G) MoBP5, (H) MoBP6, (I) MoBP7, and (J) MoBP8. As a control the respective Arabidopsis MoBP proteins were treated identically in the absence of Cnx1-E in the coincubation mixture (lane II). Biotin-labeled Cnx1-E is shown in A and F.