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. 2009 Dec 18;285(9):6725–6738. doi: 10.1074/jbc.M109.054015

FIGURE 6.

FIGURE 6.

STAT3 expression and activity in the absence of ERp57. A, RT-PCR analysis of the STAT3 mRNA in wild-type (wt), calreticulin deletion (crt−/−), ERp57−/−-ERp57ER, and ERp57−/−-ERp57cyt. B, Western blot analysis of STAT3 (inactive) and phosphorylated (Tyr705) STAT3 (active) in wild-type (wt), ERp57−/−-ERp57ER, and ERp57−/−-ERp57cyt cells. C, STAT3 activity in wild-type (wt), ERp57−/−-ERp57ER, and ERp57−/−-ERp57cyt expressing cells. ERp57−/−-GFP expressing cells were used a control. Cells were transfected with a plasmid containing the luciferase reporter gene under control of the STAT3-activated promoter. Renilla luciferase and firefly luciferase activities were measured as described under ”Experimental Procedures,“ and the relative ratio of firefly luciferase to Renilla luciferase activity in each cell lysate presented. Data are the mean ± S.D. (wild-type, n = 27; ERp57−/−, n = 21, ERp57−/−-ERp57ER, n = 12; ERp57−/−-ERp57cyt, n = 12; and ERp57−/−-GFP, n = 9.) RLU, relative light units. Two-sample, unpaired t test was performed. *, p = 0.0013 versus wild-type and #, p = 0.0001 versus wild-type. **, p = 0.0031 versus ERp57−/−-ERp57ER.