FIGURE 4.

Gpd1p is not required for β-oxidation of fatty acids. A, cell growth assay of gpd1Δ, pex3Δ, and WT cells in media with glucose or oleic acid as the carbon source. Growth was monitored using a liquid growth assay in a Bioscreen automatic reader and plotted as the average of three independent experiments and three technical replicates of each. gpd1Δ cells grew at the same rate as WT cells in oleic acid and glucose. Error bars indicate standard deviation. B, subcellular localization of ΔNGpd1p-GFP and Gpd1p-GFP-SKL in SM-Leu media containing glucose or oleic acid (8 h). C, growth assays as in A, but the strains shown are gpd1Δ cells transformed with pGPD1-GFP, pΔNGPD1-GFP, or pGPD1-GFP-SKL.