LC-ESI-MS/MS analysis of the neutral oligosaccharides on colonic mucins. ESI tandem mass spectra of [M+H]+ ions obtained from m/z 532 (A), m/z 589 (B), m/z 735a (C), m/z 735b (D), m/z 897c (E), and m/z 1043b (F), corresponding to the [M-H]− ions obtained from m/z 530, m/z 587, m/z 733a, m/z 733b, m/z 895c, and m/z 1041b in Table 1, respectively. LC-ESI-MS/MS was operated in the positive ion mode, and the collision energies applied were: for m/z 532, and 589, 5 eV; for m/z 735a, and 735b, 10 eV; and for m/z 897c and 1043b, 15 eV. Data are representative of three independent experiments.