TABLE 1.
Proteins secreted by the LEE-encoded T3SS in C. rodentium identified using SILAC-based mass spectrometry and validated by other means
| Protein name | Averaged ratioa | S.D.b | Category of secretion substrates |
|---|---|---|---|
| Proteins identified from the DBS100 WT vs. ΔescN samples | |||
| EspB | High | NAc | Translocator; effector as well? |
| EspA | High | NA | Translocator |
| Tir | High | NA | LEE-encoded effector |
| EspD | High | NA | Translocator |
| NleE | High | NA | Non-LEE-encoded effector |
| EscF | 6.2 | 1.0 | T3SS needle component |
| EspF | 5.3 | 0.9 | LEE-encoded effector |
| NleK | 3.5 | Non-LEE encoded effector | |
| rOrf8 | 3.0 | 0.6 | Component of the T3SS inner rod? |
| Proteins identified from the DBS100 ΔsepD vs ΔsepDΔescN samples | |||
| Tir | High | NA | LEE-encoded effector |
| NleA | High | NA | Non-LEE-encoded effector |
| NleE | High | NA | Non-LEE-encoded effector |
| NleH | High | NA | Non-LEE-encoded effector |
| NleC | High | NA | Non-LEE-encoded effector |
| EspG | High | NA | LEE-encoded effector |
| EspH | High | NA | LEE-encoded effector |
| EspB | High | NA | Translocator; effector as well? |
| NleB | 9.0 | 5.6 | Non-LEE-encoded effector |
| EspF | 8.9 | 2.3 | LEE-encoded effector |
| SepZ/EspZ | 8.3 | 11.4 | LEE-encoded effector |
| EspD | 8.3 | 1.7 | Translocator |
| NleG | 7.6 | 7.6 | Non-LEE-encoded effector |
| EscF | 7.6 | 0.7 | T3SS needle component |
| NleF | 6.5 | 7.4 | Non-LEE-encoded effector |
| EspX7 | 6.3 | 2.5 | Non-LEE-encoded effector |
| OspB/Ibe | 5.7 | 2.9 | Non-LEE-encoded effector |
| Map | 5.3 | 0.8 | LEE-encoded effector |
| EspM2 | 5.1 | 4.8 | Non-LEE-encoded effector |
| EspA | 4.5 | 4.2 | Translocator |
| EspT | 3.7 | Non-LEE-encoded effector | |
| NleD | 3.3 | Non-LEE-encoded effector | |
| rOrf8 | 3.2 | 0.2 | Component of the T3SS inner rod? |
a Averaged SILAC ratio (heavy isotope/light isotope) measured. “High” was given as the ratio for the type III secreted proteins that showed only heavy isotope labeled peptides in the samples.
b S.D., standard deviation as determined from quantification of several peptides when available.
c NA, not applicable.