FIGURE 5.

Interaction of TgADF with T. gondii actin filaments during sedimentation. A, effect of TgADF on the sedimentation activity of T. gondii actin filaments polymerized by the addition of F buffer. A representative Sypro-Ruby-stained SDS-PAGE gel shows the proportion of T. gondii actin sedimenting at 100,000 × g or 350,000 × g in the absence and presence of phalloidin or TgADF. Quantitation of the actin bands is indicated in the table below. p = pellet, s = supernatant. B, quantitation of the percentage of actin in the pellet fraction under the conditions in (A) based on three independent experiments, mean ± S.E.; *, p < 0.005 Student's t test, TgACT alone versus TgACT plus TgADF.