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. 2010 Feb 1;7:25. doi: 10.1186/1743-422X-7-25

Figure 1.

Figure 1

Detection of PVM RNA in potato samples by RT-PCR and confirmation of PCR products by restriction analysis. PCR amplicons (520 bp) were produced in RT-PCR using primers PVM3 and PVM4 from RNA extracted from PVM-Ca508 infected tuber (lanes 2), leaf (lane 4) and sprout (lanes 6) samples. PCR products were digested into two fragments, 370 and 150 bp with MscI (lanes 3, 5, 7) for verification. M: Molecular weight marker (100 bp DNA ladder, New England Biolabs, Pickering, Ontario); No-template control (lane 1) and negative control (RNA extracted from healthy leaves, lane 8) were used for PCR. Gel electrophoresis: 1.5% Agarose-1000 (Invitrogen Canada, Burlington, Ontario).