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Sensitivity of RT-PCR using primer set PVM3/PVM4 for detecting PVM in composite sprout (A) and dormant tuber (B) samples. RNA extracted from mixtures of infected (with PVM isolate Ca508) and healthy sprout or tuber sap at ratios of 1:0, 1:4, 1:9, 1:24, 1:49, 1:99, 1:199, 1:399, 1:799 and 0:1 (lanes 2-11, respectively). PCR amplicons: 520 bp. M: Molecular weight marker (100 bp DNA ladder, New England Biolabs, Pickering, Ontario); Lane 1: no-template control for PCR.
