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. Author manuscript; available in PMC: 2010 May 25.
Published in final edited form as: Cell. 2009 Nov 12;139(5):907–919. doi: 10.1016/j.cell.2009.10.039

Figure 1. Dynamic association of ZYG-12 with Pairing Centers during early meiosis.

Figure 1

(A) Left: Pairing Centers, marked by ZIM-1, -2, -3 and HIM-8 (red), are dispersed along the NE in transition zone nuclei. Right: the NE protein ZYG-12 (green), here detected with anti-GFP antibodies, coincides with the sites of PC association. (B) Composite images of whole gonads from wild-type, chk-2(me64), and syp-1(me17) animals, each expressing zyg-12::gfp, stained with anti-GFP (green) and DAPI (red) are shown with the distal tip oriented to the left, and meiotic progression advancing from left to right. Higher magnification views from the underlined regions (a/b) are shown to the right. In wild type animals, ZYG-12 patches appear transiently in the transition zone. At pachytene, ZYG-12 is redistributed throughout the NE. Patches are not detected in chk-2 mutants (middle), whereas the region of patches is greatly extended in syp-1 hermaphrodites (bottom) and other mutants that fail to synapse one or more chromosomes (Figure S3). Nuclei with patches also show polarized morphology by DAPI staining. Scale bars, 5µm. (C) The him-8(me4) missense mutation disrupts HIM-8 association with ZYG-12 patches. Cross sections of transition zone nuclei from him-8(me4) zyg-12:gfp animals stained with antibodies against HIM-8 (red) and GFP (green), and DAPI (blue) are shown. Scale bar, 3µm.