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. Author manuscript; available in PMC: 2011 Jan 18.
Published in final edited form as: Chem Res Toxicol. 2010 Jan;23(1):37–47. doi: 10.1021/tx9002462

Figure 1.

Figure 1

SDS-PAGE (A) and corresponding Western blot probed with monoclonal anti-HNE antibody (B) of cell lysates from THP-1 cells pretreated with 0 or 1 mM ascorbic acid for 18 h before exposure to 0 or 0.1 mM HNE for 3 h. The proteins were tentatively identified as follows: 1: Myosin-9 (MYH9-Human, MW 226,392); 2: ATP-citrate synthase (ACLY_Human, MW 120,762); 3: Heat shock protein HSP 90-β (HS90B_Human, MW 83,212); 4: Heat shock cognate 71 kDa protein (HSP7C_Human, MW 70,854); 5-1: Protein disulfide-isomerase precursor (PDIA1_Human, MW 57,081); 5-2:Pyruvate kinase isomerase M1/M2 (KPYM_Huamn, MW 57,900); 5-3: 60 kDa heat shock protein (CH60_Human, MW 61,016); 6-1: Protein disulfide isomerase A3 (PDIA3_Human, MW 56,747); 6-2: Adenylyl cyclase-associated protein 1 (CAP1_Huamn, MW 51,823); 6-3: Vimentin (VIME_Human, MW 53,619); 7: Tubulin α-1B (TBA1B_Human, MW 50,120); 8: α-Enolase (ENOA_Human, MW 47,139); 9: β-Actin (ACTB_Human, MW 41,710); 10: Fructose-bisphosphate aldolase A (ALDOA_Human, 39,395); 11: Voltage-dependent anion selective channel protein (VDAC1_Human, MW 30,754); 12-1: Carbonic anhydrase 2(CAH2_Human, MW 22,298); 12-2: 14-3-3 protein β/α (1433B_Human, MW 28,065); 13: Peroxiredoxin-6 (PRDX6_Human, MW 25,019); 14: 60S ribosomal protein L18a (RL18A_Human, MW 20,749); 15: Histone H3.1t (H31T_Human, MW 15,499); 16,17: Histone H2B type 1-M (H2B1M_Human, MW 13,981); 18: Histone H2A type 2-C (H2A2C_Human, MW 13,980); 19: Histone 4 (H4_Human, MW 11,360).