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The protein expression of TOM1L in whole lysates and immunoprecipitated lysates (anti-TOM1L antibodies) examined by western blot analysis in Jurkat cells after siRNA treatment. The siRNA experiments were performed by lenti virus-mediated infection of a hairpin construct. The expression of TOM1L was efficiently reduced in cells infected with the hairpin construct (siRNA) as compared with cells infected with a vector lacking the hairpin construct (Vector).
