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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Stem Cells. 2010 Jan;28(1):17–28. doi: 10.1002/stem.261

Figure 3. Notch inhibition by GSIs does not significantly alter cell death or clonogenic survival of irradiated T4302 CD133-negative cells.

Figure 3

T4302 CD133-negative cells were pretreated with 2 μM GSI or 0.5 μM L685,458 for 4 hours, and irradiated as indicated. (A) Relative caspase 3/7 activities were determined at 48 or 72 hour after radiation as described in figure 2B. (B) After radiation, T4302 CD133- cells in 6-well plates were cultured for three weeks, fixed and stained to count colonies. Surviving fractions were determined as described in methods.