Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Stem Cells. 2009 Oct;27(10):2393–2404. doi: 10.1002/stem.188

Figure 3.

Figure 3

Targeting IL6 receptor expression reduced GSC growth due to increased apoptosis. (A) Real-time PCR demonstrated IL6Rα expression in GSCs isolated from a D456MG human glioma xenograft was reduced via infection with lentivirus expressing two different shRNA constructs directed against IL6Rα (IL6Rα KD1 and IL6Rα KD2) when compared to lentivirus expressing a non-targeting control shRNA (NT). (B) Targeting IL6Rα via lentiviral shRNA significantly retarded the growth of D456MG GSCs as assessed with the Cell Titer Assay (Promega). Targeting IL6Rα decreases the survival of D456MG GSCs as demonstrated by Annexin V-FITC FACS analysis (C) and Caspase 3/7 activity (D). *, p < 0.01 with comparison to non-targeting shRNA. (E) Targeting IL6Rα expression attenuated the efficiency of D456MG GSCs to form neurospheres. The percentage of wells with neurospheres is indicated when infected cells were plated with ten cells per well in twenty four-well plates. (F) Representative images of neurospheres in E are shown.