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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Stem Cells. 2009 Oct;27(10):2393–2404. doi: 10.1002/stem.188

Figure 4.

Figure 4

Targeting IL6 ligand expression decreased GSC growth due to increased apoptosis. (A) Western blotting demonstrated IL6 expression in GSCs isolated from a D456MG human glioma xenograft was reduced via infection with lentivirus expressing IL6 shRNA constructs (IL6 KD1 and IL6 KD2) when compared to non-targeting control shRNA (NT). (B) Targeting IL6 via lentiviral shRNA significantly retarded the growth of D456MG GSCs as assessed with the Cell Titer Assay (Promega). (C,D) Targeting IL6 decreases the survival of D456MG GSCs as demonstrated by Annexin V-FITC FACS analysis (C) or Caspase 3/7 activity (D). *, p < 0.01 with comparison to non-targeting shRNA. (E) Targeting IL6 expression attenuated the efficiency of T3832 GSCs to form neurospheres. (F) Representative images of neurospheres in C are shown.