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. Author manuscript; available in PMC: 2010 Feb 22.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2000 Sep;41(10):3056–3060.

Figure 2.

Figure 2

RT-PCR of βB2-crystallin mRNA in human tissues. Ethidium bromide–stained gel showing the relative levels of βB2-crystallin mRNA (524 bp) after reverse transcription and amplification for 40 PCR cycles in 1 μg human lens RNA (lane 1), 6 ng purified human retina cDNA (lane 2), 3 ng purified human brain cDNA (lane 3), and 3 ng purified human testis cDNA (lane 4). Also indicated are the levels of β-actin transcripts (285 bp) in the same samples amplified under identical conditions (lanes 5 through 8). The primers for human βB2-crystallin are shown in Table 1 as oligo set 5.