Table 1.
Species | Oligonucleotide Sequence | Position | Temperature (°C) | No. Cycles |
---|---|---|---|---|
Rat | (1) F: 5′GTCACCTCGACACCAGAGAG3′ | 1–20 | 52 | 35 |
R: 5′CACTCTGGGTGTCAGGAGGA3′ | 710–691 | |||
(2) F: 5′CCACCATGGCCTCAGACCACCAGACAC3′ | 22–48 | 68 | 40 | |
R: 5′GTCCTTGTAATCCCCCTTCTCCAGCAG3′ | 545–518 | |||
(3) F: 5′CTCCGTCCTGGTGCAGGCTGG3′ | 176–196 | 54 | 30 | |
R: 5′GTAATCCCCCTTCTCCAGCAG3′ | 539–519 | |||
(4) F: 5′CCCATCAAAGTGGACAGCCAG3′ | 321–341 | 54 | 30 | |
R: 5′GTAATCCCCCTTCTCCAGCAG3′ | 539–519 | |||
Human | (5) F: 5′CCACCATGGCCTCAGATCACCAGAC3′ | 8–32 | 68 | 40 |
R: 5′GTCCTTGTAGTCTCCCTTCTCCAGCAG3′ | 531–505 |
F, forward; R, reverse.