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. Author manuscript; available in PMC: 2010 Feb 22.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2000 Sep;41(10):3056–3060.

Table 1.

Oligonucleotide Sets Used for RT-PCR Amplification

Species Oligonucleotide Sequence Position Temperature (°C) No. Cycles
Rat (1) F: 5′GTCACCTCGACACCAGAGAG3′ 1–20 52 35
  R: 5′CACTCTGGGTGTCAGGAGGA3′ 710–691
(2) F: 5′CCACCATGGCCTCAGACCACCAGACAC3′ 22–48 68 40
  R: 5′GTCCTTGTAATCCCCCTTCTCCAGCAG3′ 545–518
(3) F: 5′CTCCGTCCTGGTGCAGGCTGG3′ 176–196 54 30
  R: 5′GTAATCCCCCTTCTCCAGCAG3′ 539–519
(4) F: 5′CCCATCAAAGTGGACAGCCAG3′ 321–341 54 30
  R: 5′GTAATCCCCCTTCTCCAGCAG3′ 539–519
Human (5) F: 5′CCACCATGGCCTCAGATCACCAGAC3′ 8–32 68 40
  R: 5′GTCCTTGTAGTCTCCCTTCTCCAGCAG3′ 531–505

F, forward; R, reverse.