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. Author manuscript; available in PMC: 2010 Feb 22.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2003 Feb;44(2):672–679. doi: 10.1167/iovs.02-0018

Figure 3.

Figure 3

Ethidium bromide–stained gels showing quantitative competitive RT-PCR analysis of MTIIa induced after 8 hours of Cd2+, Cu2+, or Zn2+ treatment. RNA (300 ng) was amplified in the presence of increasing amounts (0–500 pg) of competing MTIIa mimic DNA. Indicated are the metal concentrations, the 237-bp MTIIa cDNA, the 99-bp MTIIa mimic DNA PCR products, and the calculated radioactivity incorporated in each PCR product.