Table 1.
Gene | Primer Sequence | Annealing Temperature | Product Length | Accession No. |
---|---|---|---|---|
MTIIa | AAGTCCCAGCGAACCCGCGT | 52 | 237 | J00271 |
MTIIa | CAGCAGCTGCACTTGTCCGACGC | 52 | 237 | J00271 |
MTIe | GCTCCAGCATCCCCTTTGCT | 57 | 211 | M10942 |
MTIe | CACATCAGGCACAGCAGCTG | 57 | 211 | M10942 |
MTIf | GCTTCTCTCTTGGAAAGTCC | 55 | 226 | M10943 |
MTIf | GGCATCAGTCGCAGCCGCTG | 55 | 226 | M10943 |
MTIg | GCCTCTTCCCTTCTCGCTTG | 55 | 217 | J03910 |
MTIg | GACATCAGGCGCAGCAGCTG | 55 | 217 | J03910 |
MTIh | GAACTCCAGTCTCACCTCGG | 55 | 213 | X64834 |
MTIh | GACATCAGGCACAGCAGCTG | 55 | 213 | X64834 |
αA-crystallin | CCACCTCGGCTCCCTCGTCCTAAG | 64 | 492 | NM_000394 |
αA-crystallin | CCATGTCCCCAAGAGCGGCACTAC | 64 | 492 | NM_000394 |
αB-crystallin | AGCCGCCTCTTTGACCAGTTCTTC | 60 | 452 | NM_001885 |
αB-crystallin | GCGGTGACAGCAGGCTTCTCTTC | 60 | 452 | NM_001885 |
HSP27 | CGCGCTCAGCCGGCAACTCAG | 64 | 419 | XM_055937 |
HSP27 | AGGGGTGGGCATCCGGGCTAAGG | 64 | 419 | XM_055937 |
GAPDH | CCACCCATGGCAAATTCCATGGCA | 52 | 600 | XM_006959 |
GAPDH | TCTAGACGGCAGGTCAGGTCCACC | 52 | 600 | XM_006959 |