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. Author manuscript; available in PMC: 2010 Feb 22.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2003 Feb;44(2):672–679. doi: 10.1167/iovs.02-0018

Table 1.

Primers Used for RT-PCR

Gene Primer Sequence Annealing Temperature Product Length Accession No.
MTIIa AAGTCCCAGCGAACCCGCGT 52 237 J00271
MTIIa CAGCAGCTGCACTTGTCCGACGC 52 237 J00271
MTIe GCTCCAGCATCCCCTTTGCT 57 211 M10942
MTIe CACATCAGGCACAGCAGCTG 57 211 M10942
MTIf GCTTCTCTCTTGGAAAGTCC 55 226 M10943
MTIf GGCATCAGTCGCAGCCGCTG 55 226 M10943
MTIg GCCTCTTCCCTTCTCGCTTG 55 217 J03910
MTIg GACATCAGGCGCAGCAGCTG 55 217 J03910
MTIh GAACTCCAGTCTCACCTCGG 55 213 X64834
MTIh GACATCAGGCACAGCAGCTG 55 213 X64834
αA-crystallin CCACCTCGGCTCCCTCGTCCTAAG 64 492 NM_000394
αA-crystallin CCATGTCCCCAAGAGCGGCACTAC 64 492 NM_000394
αB-crystallin AGCCGCCTCTTTGACCAGTTCTTC 60 452 NM_001885
αB-crystallin GCGGTGACAGCAGGCTTCTCTTC 60 452 NM_001885
HSP27 CGCGCTCAGCCGGCAACTCAG 64 419 XM_055937
HSP27 AGGGGTGGGCATCCGGGCTAAGG 64 419 XM_055937
GAPDH CCACCCATGGCAAATTCCATGGCA 52 600 XM_006959
GAPDH TCTAGACGGCAGGTCAGGTCCACC 52 600 XM_006959