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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Anal Biochem. 2009 Nov 11;398(1):93–98. doi: 10.1016/j.ab.2009.11.010

Fig. 4.

Fig. 4

Determination of the Michaelis constants for substrate 2 and α-chymotrypsin. Substrate 2 was incubated in reaction buffer (100 mM Tris pH 8, 10 mM CaCl2, 50 mM NaCl) with α-chymotrypsin (20 nM) for 5 minutes. The reaction was stopped by diluting the aliquots in a 20 fold excess of 8 M urea. Samples were processed as described in the experimental methods section.