Mina is a Dice1.2 candidate gene. (a) Location of chromosome 16 congenic intervals in C16D2/8 and C16D2/8D. Shown for each strain are the chromosomal regions inherited from B10.D2 (grey), BALB/c (white) or undetermined (striped). Genotyped locations are indicated with cyan dots. Genotyping markers are indicated on the right axis, with Mina in red. Long and short vertical bars indicate the original and new Dice1.2 genetic intervals, respectively. (b) Relative Il4 expression (Hprt1-normalized, arbitrary units) in TCR-activated CD4+ T cells from BALB/c (BALB), B10.D2 (D2) and C16D2/8D mice (n = 2–5; each symbol represents an individual mouse and the mean is indicated by a horizontal line). * Statistical significance was determined using a t-test; P = 0.0317. Data are representative of 2 experiments with similar results.