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. Author manuscript; available in PMC: 2010 Feb 22.
Published in final edited form as: Nat Immunol. 2009 Jun 28;10(8):872–879. doi: 10.1038/ni.1747

Figure 2.

Figure 2

Inverse correlation in Mina and Il4 expression in naïve TH cells. (a) Mina (left) and Il4 (right) mRNA expression (Actb-normalized, arbitrary units) time course from TCR cross-linking-activated CD4+ T cells isolated from low (B10.D2, C57BL/6, and C3H/HeN; filled symbols) and high (BALB/c, DBA/1J, DBA/2J, and DBA/2N; open symbols) TH2-biased strains. Data are the mean of three independent experiments and error bars indicate SEM. (b) Mina, Il4 and Ifng mRNA expression (Hprt1-normalized, arbitrary units) time course from (top) TCR cross-linking-activated BALB/c (open symbol) and C57BL/6 (closed symbol) naive TH cells or from (bottom) TH1- (closed symbol) and TH2- (open symbol) primed C57BL/6 naive TH cells. Data are representative of two independent experiments and error bars indicate SEM for triplicate PCR measurements. (c) Mina expression (Actb-normalized, arbitrary units) in CD4+ T cells stimulated with TCR cross-linking for 24 h in the presence of graded doses of IL-4 (B10.D2) or anti-IL-4 (BALB/c). Data are the mean of three independent experiments and error bars indicate SEM. (d) Mina expression (Actb-normalized, arbitrary units) in CD4+ T cells from B10.D2 mice stimulated for 24 h in the presence of anti-IL12. Data are the mean of three independent experiments and error bars indicate SEM.