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. Author manuscript; available in PMC: 2010 Feb 22.
Published in final edited form as: Nat Immunol. 2009 Jun 28;10(8):872–879. doi: 10.1038/ni.1747

Figure 5.

Figure 5

Mina can bind to and repress transcription from the Il4 promoter. (a) Identification of a Mina-responsive region in the Il4 promoter. The transcriptional response to Mina of the Il4 promoter (left; varying in length from 766 to 140 bp, indicated below the graph) and the Il2 promoter (right) is shown. Reporter activity was measured from transductants stimulated (open bars) or not (filled bars) with anti-TCR for 12 h. Data are the average of three independent experiments and error bars indicate SEM (* P < 0.05; t test). (b) Nucleotide sequence of the proximal Il4 promoter with regions (indicated as horizontal lines) used as oligonucleotide probes (A, B, C, and D) in electrophoretic mobility shift assays (EMSAs). (c) Mina interacts with the proximal Il4 promoter in EMSAs. Nuclear extracts from 24 h TCR-stimulated B10.D2 CD4+ T cells were incubated with end-labeled probes A-D in the absence (left) or presence (right, probes A and B only) of 2 μg anti-Mina. Arrows indicate the location of the Mina-containing nucleoprotein complex. Data are representative of 3 independent experiments. (d) Differential binding of Mina53 to the Il4 promoter. ChIP plot depicting fold-enrichment for chromatin-bound Mina53 at five sites (numbered relative to the transcriptional start site) across the Il4 promoter and at the Cd3e locus (as a negative control) in 24 h TCR-stimulated CD4+ T cells from strains BALB/c (open bars) and B10.D2 (filled bars). Data are the mean of five independent experiments and error bars indicate SEM (* P < 0.05, t test).