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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Nat Immunol. 2010 Jan 17;11(3):207–215. doi: 10.1038/ni.1839

Figure 2. c-Myc protein levels correlate with loss of HSC self-renewal.

Figure 2

a) FACS-dependent separation of c-Myc-eGFPHi and c-Myc-eGFPLo LSK populations. b) CFU in vitro cultures using sorted c-Myc-eGFPHi and c-Myc-eGFPLo cells. In black: First plating of LSKs, in grey: second plating. Error bars indicate standard deviation (Std) (n=3 mice in 3 independent experiments). c) Peripheral blood chimerism in competitive reconstitution assays at 25 weeks post transplant (n=3 mice). d) Relative chimerism of sorted c-Myc-eGFPHi and c-Myc-eGFPLo LSKs (CD45.2) in the bone marrow (LSK subset), thymus and spleen (d) 17 weeks post transplantation (n=3 mice).