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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Nat Immunol. 2010 Jan 17;11(3):207–215. doi: 10.1038/ni.1839

Figure 4. The role of the c-Myc:Fbw7 interaction in fetal liver stem and progenitor cells.

Figure 4

a) FACS staining defining LSK cells in e.d.14.5 fetal liver and adult (6wk old) bone marrow. b) Cell cycle status of fetal and adult LSK cells. c) Levels of c-Myc-eGFP protein expression in fetal and adult LSK cells. The overlay histogram shows induction of c-Myc protein expression in fetal LSKs. d) Levels of c-Myc-eGFP protein expression in CD150+ LSKs. e) Methylcellulose culture using the indicated cell populations purified from the fetal liver (fetal) or the bone marrow (adult). Black: first plating, Grey: second plating. Error bars indicate standard deviation (Std) (n= 6 mice). f) c-Myc-eGFPLo but not c-Myc-eGFPHi expressing fetal liver LSK subsets show chimerism in the peripheral blood 20wks post transplant in competitive reconstitution assays. CD45.2+ cells are donor-derived cells in the peripheral blood (n= 6 mice). Plots are a representation of at least 3 independent experiments.