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. 2009 Dec 8;40(3):167–183. doi: 10.1152/physiolgenomics.00148.2009

Fig. 1.

Fig. 1.

Quality control for nuclear isolation. Nuclear marker Brg1 is found only in the nuclear extract (NE) fraction. Another nuclear marker Creb1 is chiefly present in the NE fraction but is also found in the nuclear pellet (NP) fraction. Cytoplasmic marker (Alr2) is predominantly present in the cytoplasmic fractions. (Cytoplasm I = Potter-Elvehjem supernatant, Cytoplasm II = CERI/CERII supernatant.) Note that 5 μg of each sample was loaded. In this study we carried out MS analysis of the pooled Cytoplasm I and II (Cyto) as well as NE and NP fractions.