Table 6.
Transcriptional regulatory network for cholesterol biosynthesis pathway
| Transcription Factors Predicted to Regulate Cholesterol Biosynthesis Pathway | Adrenal Microarray Fold Change | Hepatic Microarray Fold Change |
|---|---|---|
| Atf2 (V$CREBP1_01) | No change | −1.20-fold |
| Atf2/Jun (V$CREBP1CJUN_01) | No change/+1.31-fold | −1.20-fold/no change |
| Cebpa (V$CAAT_01) | No change | No change |
| Cebpb (V$CAAT_01) | No change | No change |
| Cebpd (V$CAAT_01) | +1.23-fold | No change |
| Cebpg (V$CAAT_01) | No change | No change |
| Cebpz (V$CAAT_01) | No change | No change |
| Comp1 (V$COMP1_01) | No data | No data |
| Creb1 (V$CREB_01) | No change | No change |
| Dbp (V$VBP_01) | +1.43-fold | −1.47-fold |
| E2f1 (V$E2F_02) | −2.12-fold | +2.64-fold |
| E2f2 (V$E2F_02) | No data | No data |
| E2f3 (V$E2F_02) | No change | No change |
| E2f4 (V$E2F_02) | No data | No change |
| E2f5 (V$E2F_02) | No change | −1.28-fold |
| Elk1 (V$ELK1_02) | No change | No change |
| Evi1 (V$EVI1_06) | No change | No change |
| Gata1 (V$GATA1_02) | No change | No change |
| Hnf1a (V$HNF1_01) | No change | No change |
| Hnf4a (V$HNF4_01) | No change | No change |
| Hivep2/Rfx1 (V$MIF1_01) | −1.43-fold/No change | No change/No change |
| Nfya (V$NFY_Q6) | No change | −1.61-fold |
| Nfyb (V$NFY_Q6) | No change | +1.30-fold |
| Nfyc (V$NFY_Q6) | No change | No change |
| Pax6 (V$PAX6_01) | No change | +2.60-fold |
| Rfx1 (V$RFX1_01) | No change | No change |
PAINT analysis of the GenMAPP-derived cholesterol biosynthesis pathway identified a set of 26 transcription factors in the transcriptional regulatory network. The standard transcription factor gene symbol and the TRANSFAC binding matrix to which the transcription factor binds are listed; a “/” symbol indicates a transcription factor heterodimer. Fold change for the transcription factors in adrenal microarray experiments and in hepatic microarray experiments is also listed: positive fold change (+) indicates statistically significant overexpression in Chga knockout (Chga KO mean signal/wild-type mean signal); negative fold-change (−) indicates statistically significant underexpression in Chga knockout (−wild-type mean signal/Chga KO mean signal); “no data” indicates lack of a probe for the transcription factor on the microarray; “no change” indicates lack of a statistically significant fold change on the microarray.