A, effect of type I antisense (AS) on expression of the type I InsP3R in AR4-2J cells. The adenoviral construct markedly decreases expression of the type I isoform both 24 and 48 h after infection (left and right lanes, respectively), relative to uninfected control cells (ctrl). In contrast, expression of this isoform was not decreased 24 or 48 h after infection with the adenoviral DsRed construct (left and right lanes, respectively). B, expression of the type II isoform is not decreased 24 or 48 h after infection with the antisense construct for the type I InsP3R (left and right lanes, respectively), relative to uninfected control cells. C, effect of type II antisense on expression of the type II InsP3R in AR4-2J cells. The adenoviral construct markedly decreases expression of the type II isoform both 24 and 48 h after infection (left and right lanes, respectively), relative to uninfected control cells. In contrast, expression of this isoform was not decreased 24 or 48 h after infection with the adenoviral DsRed construct. D, expression of the type I isoform is not decreased 24 or 48 h after infection with the antisense construct for the type II InsP3R (left and right lanes, respectively), relative to uninfected control cells. E, effect of type III antisense on expression of the type III InsP3R in RIN cells. The adenoviral construct markedly decreases expression of the type III isoform 48 h (right) but not 24 h (left) after infection, relative to uninfected control cells. Each immunoblot was performed using 25 µg of protein/lane, and cells were infected with each adenovirus at a concentration of 5 ± 10
10 pfu/ml (m.o.i. = 40).