Table 1.
Ca2+ transients in RIN-5F cells after stimulation by ATP
| ATP (μM) | Magnitude (% increase) | Duration (s) |
|---|---|---|
| 100 | 209 ± 24 (n = 23) | 15.2 ± 0.8 (n = 31) |
| 100 (Ca2+-free) | 200 ± 13 (n = 25) | 14.8 ± 1.2 (n = 25) |
| 10 | 160 ± 14 (n = 25) | 16.8 ± 2.1 (n = 19) |
| 1 | 121 ± 15 (n = 15) | 13.0 ± 2.1 (n = 8) |
| 0.1 | 0 (n = 32) | 0 (n = 32) |
RIN-5F cells were stimulated with ATP and changes in cytoplasmic Ca2+ in individual cells were measured by Fluo-3 fluorescence using confocal line scanning microscopy. The magnitude (per cent increase over baseline) and duration of the Ca2+ transients are expressed as mean ± s.e.m. (n).